Dab tsi yogGDU?
• GDU yog Gelatin - Unit of Enzyme Viability
GDU yog Gelatin - Digesting Units. Nws yog ib chav tsev tshwj xeeb uas ntsuas qhov kev ua ntawm pineapple protease. Cias muab, qhov siab dua tus nqi ntawm GDU, ntau dua qhov muaj peev xwm ntawm bromelain kom tawg gelatin, protein substrate. Piv txwv li, ib qho GDU ntawm bromelain muaj peev xwm zom tau qee yam ntawm gelatin hauv qee lub sijhawm (piv txwv li, ib teev) raws li kev sim ntsuas.
Chav tsev no tau teeb tsa kom ntsuas kev ua haujlwm enzyme tuaj yeem ua tus qauv. Qhov no yog vim qhov kev ua ntawm ib qho enzyme plays lub luag haujlwm tseem ceeb hauv nws cov txiaj ntsig hauv ntau yam kev siv. Hauv kev tsim khoom lag luam thiab kev tshawb fawb tshawb fawb, kev ua haujlwm ntawm enzyme yuav tsum paub meej kom siv cov enzyme npaj kom tsim nyog.
• Kev sib raug zoo ntawm GDU thiab kev ua enzyme
Muaj kev sib raug zoo ntawm kev ua haujlwm ntawmpure bulk bromelain hmoovthiab GDU tus nqi. Thaum peb hais tias cov khoom lag luam bromelain muaj GDU tus nqi siab dua, nws txhais tau tias nws tuaj yeem zom cov proteins sai dua thiab ua tau zoo dua. Hauv kev ua zaub mov, piv txwv li, thaum lub sij hawm nqaij tenderisation, ib qho bromelain enzyme nrog ib tug siab GDU tus nqi lov cov myofibrillar proteins nyob rau hauv cov nqaij sai, ua rau cov nqaij mos ntau.
Los ntawm cov tshuaj kinetics pom, enzyme kev ua haujlwm ( ntsuas los ntawm GDU) txiav txim siab tus nqi ntawm cov tshuaj tiv thaiv enzyme-catalysed. Raws li Mie kab zauv (v=Vmax [S] / (Km + [S]), qhov twg v yog tus nqi ntawm cov tshuaj tiv thaiv, Vmax yog qhov siab tshaj plaws ntawm cov tshuaj tiv thaiv, [S] yog qhov concentration ntawm cov substrate, thiab Km yog Mie tas li), qhov siab dua cov enzyme kev ua haujlwm (qhov siab dua tus nqi GDU), qhov ceev ntawm cov tshuaj tiv thaiv yuav yog, yog tias muaj cov substrate txaus. Nws zoo li nyob rau hauv ib lub Hoobkas, qhov ua tau zoo dua (GDU) ntawm cov neeg ua haujlwm (enzyme), cov khoom lag luam sai dua (cov khoom tawg-down protein) yog tsim.
Cov xwm txheejAcuam tshuam rau GDU
GDU (Gelatin Digestion Unit) tus nqi ntawm bromelain qhia txog kev ua haujlwm ntawm enzyme. Nws raug cuam tshuam los ntawm ntau yam.
1. Qhov kub thiab txias
• Kev cuam tshuam ntawm qhov kub thiab txias ntawm kev ua enzyme
Bromelain zoo li feem ntau cov enzymes. Nws cov dej num muaj qhov kub thiab txias dependence. Hauv qee qhov kub thiab txias, cov haujlwm ntawm cov enzyme yuav maj mam nce ntxiv thaum qhov kub nce. Qhov no yog vim qhov kub thiab txias nce qhov thermal txav ntawm molecules. Qhov no yuav ua rau nws yooj yim dua rau substrate molecules thiab enzyme molecules kom sib tsoo nrog ib leeg, yog li ua kom ceev cov tshuaj tiv thaiv. Piv txwv li, ntawm qhov kub thiab txias, xws li 10 - 15 degree, qhov kev ua ntawm bromelain qis dua. Nws tus nqi GDU kuj tseem me me.
Qhov zoo tshaj plaws kub raupure bulk bromelain hmoovfeem ntau yog nyob ib ncig ntawm 37 - 40 degree . Hauv qhov ntsuas kub no, qhov chaw ua haujlwm ntawm cov enzyme molecule khi rau lub substrate zoo tshaj plaws. Nws tawg gelatin sai thiab ua tau zoo thiab GDU tus nqi yog qhov siab tshaj plaws. Cov tshuaj tiv thaiv catalytic ntawm cov enzyme tuaj yeem ua tau zoo thaum qhov kub thiab txias nyob ze rau tib neeg lub cev kub (37 degree). Qhov no sib raug rau ib puag ncig uas bromelain ua rau cov kab mob nyob.
• Cov teebmeem ntawm qhov kub thiab txias
Thaum qhov kub siab tshaj qhov ntsuas kub zoo tshaj plaws, cov haujlwm ntawm cov enzyme txo qis heev. Qhov no yog vim qhov kub thiab txias ua rau denaturation ntawm enzyme proteins. Lub spatial qauv ntawm enzymes proteins yog qhov tseem ceeb rau lawv txoj haujlwm. Qhov kub ntau dhau ua rau cov txheej txheem theem nrab thiab theem nrab ntawm cov enzyme molecule. Qhov no ua rau muaj kev hloov pauv hauv qhov chaw ua haujlwm, uas poob nws lub peev xwm los khi substrates thiab catalyze cov tshuaj tiv thaiv. Piv txwv li, thaum kub nce mus txog 60 - 70 degree , bromelain tej hmoov yuav raug mob hnyav denaturation thiab tus nqi GDU yuav luag zero.
2. pH Factor
• Kev cuam tshuam dav dav ntawm pH ntawm kev ua enzyme
Cov haujlwm ntawm bromelain kuj tseem cuam tshuam los ntawm pH. Kev sib txawv pH ib puag ncig yuav hloov lub xeev tus nqi ntawm cov enzyme molecule lub chaw ua haujlwm thiab cov enzyme protein ua raws. Hauv qee qhov pH ntau, enzyme kev ua haujlwm sib txawv nrog pH. Feem ntau hais lus, bromelain qhia tau hais tias kev ua haujlwm zoo dua hauv pH ntau ntawm 6 - 8, nrog GDU qhov tseem ceeb.
Nyob rau hauv no pH ntau, qhov dissociation ntawm acidic thiab yooj yim amino acid residues nyob rau hauv lub enzyme molecule yog haum rau tsim ntawm qhov tseeb enzyme-substrate complex nrog lub substrate. Piv txwv li, nyob ib ncig ntawm pH 7, qhov chaw nquag ntawm cov enzyme molecule tuaj yeem cuam tshuam zoo nrog cov peptide bonds hauv gelatine molecule, pab txhawb cov tshuaj tiv thaiv hydrolysis.
• Cov teebmeem ntawm pH siab lossis qis
Kev ua enzyme txo qis qis ntawm pH qhov tseem ceeb hauv qab 6 lossis siab dua 8. Enzyme proteins tuaj yeem ua rau tsis zoo nyob rau hauv ib puag ncig acidic lossis alkaline dhau lawm. Piv txwv li, nyob rau hauv ib puag ncig acidic heev (pH <4), cov amino acid tseem ceeb nyob rau hauv cov enzyme molecule yog overpronated, ua rau muaj kev hloov pauv hauv cov enzyme protein, kev puas tsuaj ntawm lub chaw ua haujlwm thiab txo qis GDU tus nqi. Ib yam li ntawd, nyob rau hauv ib puag ncig alkaline overly alkaline (pH> 9), acidic amino acid residues yuav overly dissociated, uas yuav ua rau lub enzyme poob nws cov hauj lwm.
3. Substrate concentration factor
● Cov nyhuv ntawm substrate concentration ntawm enzyme kev ua
Thaum lub substrate concentration yog tsawg, nrog rau kev nce substrate concentration, lub sij hawm rau lub enzyme ua ke nrog lub substrate nce. Cov tshuaj tiv thaiv tus nqi yuav nrawm dua thiab GDU tus nqi yuav nce ntxiv. Piv txwv li, thaum gelatin concentration yog nce los ntawm 0.5% mus rau 1%,pure bulk bromelain hmoovmuaj ntau substrate los ua, qhov ua tau zoo ntawm gelatin decomposition yog nce, thiab GDU tus nqi nce.
• Cov nyhuv ntawm cov substrate siab concentration
Thaum lub substrate concentration siab dhau lawm, txoj kev loj hlob ntawm enzyme kev ua si yuav maj mam qeeb. Qhov no yog vim tus naj npawb ntawm cov chaw ua haujlwm ntawm cov enzyme raug txwv. Thaum lub substrate concentration nce mus txog ib theem, tag nrho cov active chaw ntawm lub enzyme yog nyob rau hauv lub substrate molecules, thiab nce lub substrate concentration yuav tsis ho nce cov tshuaj tiv thaiv tus nqi, thiab GDU tus nqi tseem nyob ruaj khov.
Qhov tseem ceeb ntawm GDU hauv ntau qhov chaw
• Pharmaceuticals
Hauv cov tshuaj, bromelain muaj cov tshuaj tiv thaiv kab mob thiab tiv thaiv o. Nws tus nqi GDU yog qhov tseem ceeb heev hauv kev txiav txim siab cov tshuaj noj. Piv txwv li, thaum kho mob tom qab mob o lossis mob o, GDU tus nqi ntawm bromelain yog qhov xav tau los xam qhov tseeb ntawm cov enzyme yuav tsum tau muab rau tus neeg mob. Qhov no yog tib txoj hauv kev kom ua tiav cov txiaj ntsig kho tau zoo thaum tsis txhob muaj kev phiv los ntawm kev ua haujlwm enzyme ntau dhau.
• Kev lag luam zaub mov
Hauv kev lag luam zaub mov, xws li nqaij tenderisation tau hais ua ntej. Cov khoom sib txawv thiab cov txheej txheem yuav xav tau GDU qhov tseem ceeb ntawm bromelain. Yog tias tus nqi GDU qis dhau lawm, qhov kev xav tau ntawm kev sib tw yuav tsis ua tiav. Yog tias nws siab dhau lawm, nws tuaj yeem rhuav tshem cov protein ntau dhau, ua rau poob ntawm elasticity thiab tsw qab. Tsis tas li ntawd, ntshiab bromelain hmoov yog siv hauv npias tsim los tiv thaiv huab cua txias ntawm npias. Bromelain ntawm txoj cai GDU tus nqi lov tawm turbidity-ua rau cov proteins, txhim kho kev ruaj ntseg thiab meej ntawm npias, thiab ncua lub txee lub neej ntawm npias.
Guanjie Biotech yog cov khoom lag luam bromelain ntau, peb tsim 2400GDU bromelain hmoov. Peb cov khoom dhau HALAL, Kosher, HACCP, ISO9001 thiab lwm yam patents. Siab zoo. Yog tias koj xav yuav bromelain hmoov, txais tos nug peb:info@gybiotech.com.
