Sulforaphane Sulforaphane nyob rau hauv Broccoli sprouts?

Broccoli sprouts tau txais kev saib xyuas tseem ceeb nyob rau hauv xyoo tsis ntev los no raws li kev nplua nuj ntawmCov ntshiab suloraphane, lub zog ua kom muaj zog bioctive yog rau cov pab pawg othiocyanananananananate pab pawg, uas tau muab los ntawm glucoraphanin, yog glucosinolate precursor. Sulernane tau kawm rau nws cov txiaj ntsig kev noj qab haus huv, suav nrog cov tshuaj tiv thaiv kev noj qab haus huv, tiv thaiv kev mob qog nqaij hlav, thiab neuroprotective cuam tshuam.

Nkag siab ntau npaum li cas sulforaphane yog tam sim no hauv zaub cob pob yog qhov tseem ceeb rau cov kws tshawb nrhiav, thiab cov neeg siv khoom ua kom muaj txiaj ntsig zoo. Kab lus no muab cov ncauj lus kom ntxaws ntxaws txog sulorganes cov ntsiab lus hauv cov zaub cob pob, cov yam cuam tshuam nws theem, thiab kev cuam tshuam cuam tshuam.

Sulforaphane yog dab tsi?

Cov kua ntshiab sulforaphane (cov mis nyuj: C6h11NOS2) yog ib qho kev sib txuas lus zoo li cov zaub mov ua ke xws li zaub mov noj qab zib, pob txha caj dab, kale, thiab zaub qhwv. Zaub cobholi tau muaj tshwj xeeb yog theem siab ntawm glucorapanin thiab yog li sawv cev rau qhov muaj peev xwm ntawm cov sulforaphane.

Sulforaphane tsis muaj nyob hauv cov zaub tsis huv; Nws yog tsim tsuas yog thaum cog cov ntaub so ntswg puas (piv txwv li, zom, chopping) kom qhib myrosinase.

Sulforaphane cov ntsiab lus hauv zaub cob pob zaub tawg

Raug sulforaphale concentrations

Zaub cob pob nrov nrov yog feem ntau hais txog li muaj nyob txhua qhov chaw ntawm 20 txog 50 zaug ntau glucorapaRapaRapaRapanin dua li cov kab mob broccoli ntau dua. Vim tias sulforaphane yog muab tau los ntawm glucorapanin, qhov no cuam tshuam ntau dua cov peev txheej sulforaphane paib.

Cov kev tshawb fawb ntau tshaj plaws tau ntsuas sulforaphane cov ntsiab lus hauv ntau theem ntawm kev loj hlob, feem ntau yog lub sijhawm sau qoob loo rau cov lag luam brocololi sprouts.

• Cov ntsiab lus glucorapanin:

Zaub cob pob muaj kwv yees li 0. 1% rau 1.5% suloraphane los ntawm qhov hnyav thiab kev loj hlob thiab kev loj hlob kev loj hlob. Qhov no ntxhib equates rau 1 txog 15 mg ntawm glucoraphanin ib gram ntawm qhov hnyav.

• Sulforaphane tawm:

Thaum myrosinease yog nquag thiab hloov dua siab tshiab, sulforaphane paib muaj ntau yam los ntawm qhov hnyav hauv cov zaub cob pob hauv cov zaub cob pob.

Cov nqi raug los ntawm cov ntawv nyeem

Cov kev ntseeg no tuaj yeem sib txawv ntau vim muaj ntau yam kev sib tham hauv qab no.

Yam cuam tshuam rau Sulforaphhane cov ntsiab lus hauv Broccoli sprouts

Cov Sulforaphane, tsis muaj biologically muaj calothiocyanate, tsis muaj nyob rau hauv cov zaub cob pob tab sis yog tsim los ntawm kev ua yeeb yam ntawm glucorapanin los ntawm myrosinase thaum cog cov ntaub so ntswg puas puas. Raws li xws li, sulelaphane cov ntsiab lus hauv zaub cob pob zaub qhwv tuaj yeem sib txawv nyob ntawm ntau intrinsic thiab extrinsic yam. Hauv qab no yog cov ncauj lus kom ntxaws sib tham ntawm ntau yam uas cuam tshuam thiab bioavailability thiab bioavailability ntawm cov ntshiab sulforaphane hauv zaub cob pob.

Genetic thiab cultivar txawv

Cov zaub mov txawv txawv cultivars ib txwm muaj ntau yam ntawm glucoraphanin, tus sulforaphane precursor. Txoj kev sib txawv ntawm caj ces yog ib qho ntawm feem ntau ntawm cov feem ntau cuam tshuam txog sulforaphane paib.

High-glucoraphin cultivars xws li Brassica likeracea v. Italica tau xaiv bred los muaj ntau glucorapanin.

Muaj qee kis, Super Sprout cultivars tej zaum yuav muaj txog li 100 mg ntawm glucoraphanin ib 100-50 mg ntawm ntshiab li 30-50 mg ntawm ntshiab suloraphane yog tias kev hloov pauv yog qhov zoo tshaj plaws.

Cov kev tshawb fawb tau qhia ib qho 10-} muting variation nyob rau hauv glucoraphin cov ntsiab lus thoob plaws cultivars, cuam tshuam ncaj qha cuam tshuam sulforaphane paib.

Noob Khoom Zoo Thiab Ntim

Noob neeg muaj kuab lom thiab kev cia khoom cia kuj tseem ceeb tej yam ntshiab suloraphane cov ntsiab lus hauv sprouts.

Cov noob khaws cia nyob rau hauv cov av noo lossis nce qhov kub tuaj yeem dhau los ua tsis tau kev nyuaj siab txog glucosinolates, tshwj xeeb yog glucorapanin.

Cov noob nyoog muaj hnub nyoog zoo li muaj cov kab mob ua kom qis dua thiab yuav ua rau Broccoli Sprout extract sulforaphane nrog txo biosSyncanin.

Lub sijhawm germination thiab sprout muaj hnub nyoog

Cov ntsiab lus suloraphane suloraphane txawv nyob tseem ceeb nyob ntawm lub hnub nyoog ntawm sprouts.

• Hnub 1-2:

Tsawg suloraphane qib vim ua tiav ua tsis tiav glucorapapanin synthesis.

• Hnub 3-5:

Peak Suloraphane ntau lawm; Myrosinase kev ua haujlwm yog qhov siab, thiab cov roj glucorapanin yog qhov zoo.

• hnub 6-7 thiab dhau:

Sulforaphane yuav poob raws li cov qe pib ua kom tiav thiab hloov metabolic tsom.

Kev tshawb fawb qhia tias {3- hnub ci sprouts tau tawm ntawm qhov zoo tshaj plaws ntawm cov ntshiab sulforaphe cuam tshuam rau qhov hnyav. Tom qab 5 hnub, glucorapanin qib yuav ua rau cov nplooj ntuj los yog poob vim kev sib txawv lossis kev sib txawv ntawm tes.

Lub teeb kis thaum muaj kev kub ntxhov

Lub teeb yog lwm qhov tseem ceeb tseem ceeb hauv kev txiav txim siab zaub cob pob Broccoli hmoov sulforaphane thiab, los ntawm kev txuas ntxiv, sulforaphane tawm los.

Cov xwm txheej tsaus nti yuav ua rau muaj kev tsim ntawm qee yam glucosinolates, suav nrog glucorapanin.

Kev tsim kho lub teeb nruab nrab (12-16 teev

Txawm li cas los xij, ntau lub teeb yuav cov kev ntxhov siab los yog txo cov biomass, ua rau cov kev lag luam tawm-offs.

Kub thiab av noo thaum caij qe

Ib puag ncig tsis xws li qhov kub thiab cov av noo hais cov haujlwm biosynthesis thiab enzymatic ua haujlwm koom nrog sulelaphane hmoov ntau lawm.

• Muaj qhov ntsuas kub ntawm qhov ntsuas kub: 20-25 degree (68-77 degree f). Qhov no txhawb nqa lub zog loj hlob thiab enzymatic efficiency.

•Higher temperatures (>30 Qib) tuaj yeem ua rau kev loj hlob sai dua tab sis txo qis glucoraphan ntxiv thiab ntxhov siab.

• Thaum kub qis (<15°C) may reduce germination rates and myrosinase activity.

Cov av noo yuav tsum muaj siab txaus (90-95%) los txhawb tawm qe tab sis tsis yog li ntawd kom muaj kev sib kis fungal lossis kev ua haujlwm ua ntej ntawm glucorapanin. Cov xwm txheej zoo tagnrho yog ib puag ncig 22-25 degree thiab 90-95% tus txheeb ze cov av noo.

Txoj kev ua (kev lag luam thiab hauv kev lag luam)

Cov tswv yim sib txawv tuaj yeem hloov kho cov ntshiab sulforaphane muaj:

• blanching lossis boiling:

Ua kom puam myrosinase thiab txo sulforaphane tau tawm los ua kom zoo.

• Tso (1-3 feeb):

Inactivates esp thaum khaws cov myrosinase, nce sullocane tsim.

• Kom qhuav (khov-kom qhuav, huab cua qhuav):

Yuav txo cov dej dej thiab mloog zoo glucorapanin, tab sis tej zaum yuav muaj kev sib haum xeeb enzymatic tshwj tsis yog ua raws li kev tswj hwm.

• Fermentation lossis enzymatic ua ntej kev kho mob:

Ib co broccoli extract sulforaphana cov khoom lag luam siv cov microbial fermentation kom pab txhawb kom muaj kev vam meej ntawm endogenous myrosinase.

Kev ua kom tsawg heev lossis kev tswj hwm enzyme tswj tau tus yuam sij los tuav lossis txhim kho sulforaphane qib.

Cov hau kev rau kev ntsuas sulforaphane cov ntsiab lus

Kev Ntsuam Xyuas Cov Ntxhi

Kev ua haujlwm siab ua kua chromatography (HPLC) nrog UV lossis MS Nrhiav tau yog cov qauv rau cov glucoraphanin thiab sulforaphane.

Roj Chromatography-Pawg Spectrometry (GC-MS) yog siv rau kev hloov tawm italiocyanates.

Yog qhov tseeb ntawm sulforapanane ntsuas yuav tsum tau siv cov dej num kom huv si hauv kev tswj hwm kom mimic zom.

Cov txheej txheem Extraction

Cov Sulforaphane yog rho tawm los ntawm cov tshiab lossis khov-qhuav sprouts.

Cov kauj ruam hloov zuj zus yog qhov tseem ceeb rau kev ntsuas qhov ntsuas sulloforaphane muaj peev xwm ntau dua li glucorapanin cov ntsiab lus ib leeg.

Zaub cob pob yog qhov tshwj xeeb nplua nuj ntawm sulforaphane s vim tias lawv cov siab glucorapaRapanin cov ntsiab lus thiab cov khoom siv tau ua haujlwm myrosinase. Cov ntsiab lus suloraphanes sib txawv txawv raws li cov kev sib cais ntawm caj ces, ib puag ncig, feem ntau yog ib yam dab tsi los ntawm {}}. 2 txog 0.5 mg \/ g mis tshiab.

Peb pib los ntawm kev ua tib zoo xaiv cov noob los ntawm 138 broccoli ntau yam los xyuas kom meej cov khoom siv raw zoo tshaj plaws. Tom ntej no, peb siv cov thev naus laus zis thev naus laus zis thev naus laus zis los txhim kho cov ntshiab sulforaphane paib. Peb qhov tsawg-qhov kub thiab siv hluav taws xob tsawg khaws cov khoom xyaw bioactive, ua rau cov khoom lag luam nrog cov khoom ruaj khov thiab txhim kho ruaj khov. Txhua lub batch ntawm zaub cob pob ntxiv sulforaphane yog kev sim nruj thiab ntim khoom zoo los tswj cov ntshiab suloraphane zoo. Yog tias koj xav paub txog kev kawm ntau dua, tiv tauj peb ntawminfo@gybiotech.com.