Yog lawm,Urolithin UA, lub ntuj polyphenolic compound tsim los ntawm plab microbiota los ntawm cov metabolism ntawm ellagic acid thiab ellagitannins, tau txais kev saib xyuas tseem ceeb hauv kev tshawb fawb txog lub neej nyob rau xyoo tas los no. Raws li cov pov thawj scientific uas twb muaj lawm, cov txheej txheem molecular thiab cov ntaub ntawv ua tau zoo ntawm cov ntshiab Urolithin A raws li cov tshuaj antioxidant, nrog rau nws qhov kev thov dav dav hauv kev lag luam raw khoom, lav kev tshawb nrhiav ntxiv.
Dab tsi yogTshuajCov khoomntawm Urolithin A?
Urolithin A muaj cov tshuaj formula C₁₃H₈O₄ thiab CAS naj npawb 1143-70-0. Nws yog polyphenolic metabolite nrog dibenzo [b,d] pyran-6-ib pob txha. Chemically, nws belongs rau qeb ntawm bioactive natural khoom. Nws cov precursors feem ntau pom nyob rau hauv cov nroj tsuag xws li pomegranates, txiv ntseej, thiab berries. Tib neeg lub cev tsis tuaj yeem nqus urolithin ncaj qha los ntawm qhov chaw ntuj. Hloov chaw, cov kab mob hauv plab hnyuv hloov ellagic acid rau hauv urolithin A. Vim yog qhov sib txawv ntawm cov plab hnyuv microbiota, qhov ua tau zoo ntawm kev hloov cov khoom noj uas muaj ellagic acid nplua nuj rau hauv urolithin A txawv ntawm cov tib neeg. Qhov kev hloov pauv no tsim lub hauv paus rau kev lag luam rationale qab exogenous urolithin A supplementation.
Yog UrolithinA Ib qho Antioxidant?
Yog lawm, urolithin A yog ib qho antioxidant.
Direct Dawb Radical Scavenging Peev Xwm ntawm Urolithin A
Nyob rau hauv vitro dawb radical scavenging thwmsim muab ntau cov pov thawj rau kev ntsuam xyuas qhov ncaj qha antioxidant muaj peev xwm ntawm cov tebchaw. Siv cov txheej txheem oxygen radical absorbance peev xwm (ORAC), cov tshuaj tiv thaiv antioxidant ntawm cov urolithin A yog sib npaug rau 13.2 μM Trolox. Hauv kev sim superoxide anion dawb radical scavenging, ib nrab -qhov siab tshaj plaws inhibitory concentration (IC₅₀) ntawm urolithin A yog 5.01 μM. Hauv kev sim DPPH dawb radical scavenging, IC₅₀ yog 152.66 μM.
Cov ntaub ntawv no qhia tias urolithin A tuaj yeem cuam tshuam ncaj qha nrog cov dawb radicals. Los ntawm cov qauv molecular foundations, phenolic hydroxyl pawg nyob rau hauv lub molecule yog cov active chaw uas muab hydrogen atoms. Qhov no yog lub hauv paus txheej txheem rau qhov ncaj qha antioxidant kev ua ntawm polyphenolic tebchaw. Txawm li cas los xij, piv nrog qee cov tshuaj muaj zog antioxidants, hauv vitro dawb radical scavenging kev ua si ntawm ntshiab urolithin A tsis yog nws lub ntsiab feature. Kev tshawb fawb qhia tias cov teebmeem hauv vivo antioxidant ntawm cov tshuaj no nyob ntawm ntau dua ntawm nws txoj haujlwm tswj hwm hauv cellular antioxidant network dua li ntawm cov dawb radical neutralization yooj yim.
Kev tswj lub luag haujlwm ntawm Urolithin A hauv Antioxidant Enzyme Kev Ua Haujlwm
• Kev cai ntawm SOD2 thiab Mitochondrial Reactive Oxygen Species Scavenging
Superoxide dismutase 2 (SOD2) yog ib qho tseem ceeb antioxidant enzyme nyob rau hauv mitochondrial matrix uas yog lub luag hauj lwm rau converting superoxide anions rau hauv hydrogen peroxide. Hauv kev sim siv vascular du leeg hlwb los ntawm spontaneously hypertensive nas, kev kho mob nrog urolithin A (25 μM) txo qis mitochondrial reactive oxygen hom (ROS) qib thiab txhim kho SOD2 kev ua.
Cov kev tshawb fawb Mechanistic tau qhia tias urolithin A hmoov txhawb kev tsim cov isoform luv ntawm SIRT3 (SL-SIRT3) hauv mitochondria, yog li kho cov deacetylation ntawm SOD2. Deacetylated SOD2 qhia txog kev ua kom muaj zog ntau dua. SIRT3 inhibitor 3-TYP tshem tawm cov kev tswj xyuas cov teebmeem ntawm urolithin A ntawm SOD2 deacetylation, mitochondrial ROS theem, thiab cell proliferation thiab tsiv teb tsaws. Cov kev tshawb pom no tsim ib txoj hauv kev ua tiav: urolithin A → SIRT3 → SOD2 deacetylation → mitochondrial ROS txo.
Hauv kev sim tsiaj, kev txhaj tshuaj intraperitoneal ntawm urolithin A (50 mg / kg txhua 2 hnub rau 4 lub lis piam) hauv cov nas uas muaj ntshav siab nce ntxiv SL-SIRT3 qib thiab SOD2 kev ua haujlwm hauv cov hlab ntsha aorta thiab mesenteric thaum txo qis SOD2 acetylation thiab mitochondrial ROS theem. Cov ntaub ntawv no tau lees paub tias urolithin A tuaj yeem tswj hwm kev ua haujlwm antioxidant enzyme hauv vivo.
• Txhim kho CAT, GPx, thiab GR Kev Ua Si
Hauv Neuro-2a cell oxidative stress model, pretreatment nrog urolithin A (0.5-4 μM), tom qab ntawd raug rau 250 μM hydrogen peroxide, txhim kho catalase (CAT), glutathione peroxidase (GPx), thiab glutathione reductase (GR) kev ua ub no. Tib lub sijhawm, urolithin A tau tswj hwm qhov kev qhia ntawm peroxiredoxin 1 (Prdx1) thiab peroxiredoxin 3 (Prdx3), ob qho tib si ua lub luag haujlwm tseem ceeb hauv kev tshem tawm hydrogen peroxide.
• Ua kom muaj NRF2 Txoj Kev
NRF2 (nuclear factor erythroid 2-related factor 2) yog ib qho tseem ceeb ntawm kev hloov pauv uas tswj cov cellular antioxidant teb. Raws li ib txwm muaj lub cev muaj zog, NRF2 khi rau KEAP1 hauv cytoplasm thiab dhau mus rau ubiquitin-mediated degradation. Thaum ua kom muaj zog, NRF2 hloov mus rau lub nucleus, khi rau cov ntsiab lus teb antioxidant (AREs), thiab pib cov kev hloov pauv ntawm cov noob caj noob ces antioxidant.
Hauv UVA-induced photoaging qauv siv tib neeg dermal fibroblasts, urolithin A hmoov txhawb nqa cov phosphorylation thiab nuclear translocation ntawm NRF2, yog li ua kom cov enzymes qis qis. Qhov no mechanism synergizes nrog induction ntawm mitophagy los ntawm urolithin A, sib koom ua ke kho cov teebmeem cytoprotective.
Inhibitory Effects ntawm Urolithin A ntawm Oxidase Activity
Lwm lub tswv yim los txo oxidative kev nyuaj siab yog inhibit enzymes uas tsim reactive oxygen hom (ROS). Cov kev tshawb fawb tau pom tias urolithin A nthuav tawm ib koob tshuaj - nyob ntawm inhibitory nyhuv ntawm monoamine oxidase A (MAO-A) thiab tyrosinase. MAO-A, nyob rau sab nraud mitochondrial membrane, catalyzes cov tshuaj tiv thaiv uas tsim hydrogen peroxide raws li cov khoom lag luam. Los ntawm inhibiting cov enzyme no cov haujlwm, urolithin A tuaj yeem txo cov mitochondrial ROS tiam.
Tsis tas li ntawd, nyob rau hauv vascular du leeg hlwb, urolithin A (25 μM) inhibits NADPH oxidase (NOX) kev ua thiab downregulates NOX1 qhia, tab sis tsis muaj qhov tseem ceeb ntawm NOX2 thiab NOX4 qhia. NADPH oxidase yog ib qho ntawm cov ntsiab lus tseem ceeb ntawm superoxide anions hauv hlwb, thiab inhibiting qhov kev ua enzyme no ntxiv rau cov multi-txoj kev antioxidant mechanism ntawm urolithin A hmoov.
Ib txoj kev tshawb fawb tseem ceeb luam tawm nyob rau hauv Phau Tsom Faj Txog Kev Ua Liaj Ua Teb thiab Khoom Noj Khoom Noj los ntawm Kallio pab neeg hauv 2013. Txoj kev tshawb no qhia tias cov khoom siv redox ntawm urolithin A nyob ntawm qhov kev kuaj pom:
• Organic oxidative assay (ORAC):
Urolithin A nthuav tawm cov kev ua haujlwm antioxidant muaj zog heev.
• Cellular- raws li kev soj ntsuam:
Thaum tsis muaj kev ntxhov siab, urolithin A tuaj yeem nthuav tawm me me pro-oxidative zog nyob rau hauv cov hlwb, thaum nws niam nws txiv compound, ellagic acid, tsis qhia cov tshuaj tiv thaiv no.
Yuav Ua Li Cas NtxivUrolithin Arau Formulation?
Raws li polyphenolic compound, urolithin A hmoov yuav tsum tau tsim kom tsis txhob muaj zog alkaline thiab oxidizing ib puag ncig. Microencapsulation los yog liposome encapsulation yog pom zoo los txhim kho nws txoj kev xa khoom hauv plab hnyuv. Guanjie Biotech's bulk urolithin A hmoov muaj siab purity thiab zoo flowability, ua rau nws haum rau ntsiav tshuaj, tshuaj ntsiav, thiab hmoov ntau lawm kab.
Xaus:
Hauv cov ntsiab lus, cov nyhuv antioxidant ntawm urolithin A UA yog ua raws li lub tswv yim triple: ncaj qha scavenging ntawm cov dawb radicals, ua kom cov Nrf2-ARE antioxidant txoj hauv kev, thiab tseem ceeb txo cov oxidative stressors los ntawm inducing mitophagy. Cov pov thawj preclinical tam sim no txhawb nqa nws cov kev siv peev xwm hauv kev kho mob vascular remodeling, neuroprotection, thiab tiv thaiv kev laus.
Rau cov neeg siv B2B nrhiav cov khoom sib txawv, urolithiasis A's mechanism ntawm kev ua txawv ntawm cov khoom noj khoom haus ib txwm muaj. Nws sawv cev rau tiam tom ntej ntawm cov khoom siv raw uas tsom mus rau mitochondria. Nws raug nquahu kom cov neeg yuav khoom tsom mus rau purity, microbial stability, thiab hnyav hlau tswj cov qauv ntawm cov khoom lag luam thaum xaiv cov khoom siv urolithin A ntau.
Cov ntaub ntawv:
[1] Urolithin A alleviates vascular remodeling los ntawm mitochondrial SIRT3-mediated SOD2 deacetylation thiab antioxidation nyob rau hauv cov nas hypertensive. Redox Report, 2026, 31(1): 2622255.
[2] Urolithin A (3,8-dihydroxy-6H-dibenzopyran-6-one). Abmole Bioscience.
[3] Mechanistic Insights rau Kev Siv Tshuaj lom neeg thiab Kev Ua Haujlwm Antioxidant ntawm Walnut Ellagitannins: Kev Ntsuam Xyuas Txheej Txheem. Antioxidants, 2024, 13(8): 974.
[4] Cov Metabolite Urolithin-Ameliorates Oxidative Stress nyob rau hauv Neuro-2a Cells, dhau los ua Tus Neeg Muaj Peev Xwm Neuroprotective. Antioxidants, 2020, 9(2): 177.
[5] Kev tshawb fawb nce qib ntawm cov tshuaj pharmacological thiab cov txheej txheem ntawm urolithiasis A. Central South Pharmacy, 2022, 20(1).
[6] Lub hom phiaj kev laus nrog urolithin A hauv tib neeg: Kev tshuaj xyuas zoo. Kev Tshawb Fawb Kev Laus, 2024, 100: 102406.
[7] Cov teebmeem ntawm Urolithin Ib qho ntxiv ntawm kev ua tau zoo thiab cov tshuaj tiv thaiv antioxidant hauv kev ncaws pob ncaws pob thaum lub caij ua ntej: kev sim randomized tswj. DOAJ, 2025.
[8] Urolithin A tiv thaiv tib neeg dermal fibroblasts los ntawm UVA-induced photoaging los ntawm NRF2 activation thiab mitophagy. J Photochem Photobiol B, 2022.
