Tej Hyaluronic Hyaluronic Acidyog qhov dav hauv cov tshuaj pleev ib ce, nutraceuticals, phais mob ophthalmic, qhov txhab zoo, thiab cov khoom siv kho mob. Txawm li cas los xij, vim nws hygroscopic thiab biodroscopic thiab biodroscopic thiab biodegradable Xwm, rooj thiab khaws cia ntawm ha yog qhov tseem ceeb kom ntseeg tau tias nws qhov ruaj khov thiab kev ua tau zoo. Guanjie Biotech yog ib qho loj heev acid xa khoom. Peb muab siab - Zoo hyaluronic acid uas tau raws li kev xav tau ntawm ntau yam kev lag luam. Cia peb saib li cas Hyaluronic acid yuav tsum tau muab cia thiab seb Hyaluronic Acid muaj lub txee txee.
Cov Khoom Siv Physicochemical ntawm Hyaluronic Acid
Nkag siab cov qauv molecular ntawm ntau ntau hyaluronic acid yog qhov tseem ceeb kom txiav txim siab nws cov kev cia. Ha yog lub siab molecular hnyav polysaccharide muaj los ntawm rov qab ua kom muaj Disaccharide Units N} acetyllucosamine thiab d {{2} glucuronic acid. Nws yog hydrophilic thiab cov ntaub ntawv heev viscous cov kev daws teeb meem, ua rau nkag siab noo noo, Ph, qhov kub thiab txias thiab thiab cov microbial.
Cov yam ntxwv tseem ceeb cuam tshuam rau kev cia khoom:
• Helgroscopic:
Tej ntau hyaluronic acid nqus dej tau yooj yim los ntawm huab cua.
• Hydrolyzable: Raug rau cov hydrolysis ntawm cov tsiaj heev.
• biodegradable: decomposed los ntawm hyaluronidase enzymes thiab cov kab mob.
• Thim samkas: tuaj yeem txwv tsis taus ntawm cov kub.
• Oxidation rhiab: degrades nyob rau hauv muaj cov dawb radicals lossis UV.
TxawvCov Ntawv Ntawm Hyaluronic Acid Chaw ntim khoom Hyaluronic acid hmoov
Ntau ntau cov hmoov acid hmoov yog qhov ruaj khov tshaj plaws thiab siv tau siv hauv kev tsim khoom. Nws zoo nkaus li lub ntsej muag dawb rau tawm - cov hmoov dawb, tsw ntxhiab thiab muaj lub neej ntev ntev thaum khaws cia kom raug.
Cov Lus Qhia Cia Rau Ha Hmoov:
• Ntsuas kub:
Rau kev ua kom zoo tshaj plaws khaws cia, khw His hmoov ntawm 2 qib mus txog 10 degree. Chav nyob kub (nyob ib ncig ntawm 25 degree) yog tau txais rau luv- lub sij hawm tuav tab sis tsis zoo tagnrho rau kev ncua ntxiv. Zam kev ua kom sov lossis raug rau qhov kub thiab txias saum toj no 30 degree, uas yuav ua kom nrawm nrawm degradation.
• Cov av noo:
Hyaluronic acid loj hmoov yog hygroscopic zoo. Raug rau cov av noo siab tuaj yeem ua rau ua kom tsis taus thiab txo qis. Saib xyuas cov av noo hauv qab 30%. Ib txwm khaws cia rau hauv ib puag ncig qhuav.
• Lub Teeb Raug:
Ha degrades rau teb rau UV teeb thiab ncaj qha tshav, uas tuaj yeem pib pib oxidatatural cov kev tawm tsam. Khaws rau hauv qhov tsaus ntuj, opaque ntim, deb ntawm lub teeb.
• Ntim:
Siv airtight, noo noo - ntawv pov thawj, thiab lub teeb {1} {{{{1 li aluminium ntawv ci lossis HDPE ntim. Desiccants yuav tsum tau ntxiv rau nqus dej noo thaum cia thiab thauj khoom.
• Txee lub neej:
Thaum khaws cia rau hauv qhov chaw txias, qhuav, thiab tsaus ib puag ncig, ntau cov hmoov acid hmoov tuaj yeem tswj tau nws qhov zoo rau li 2-3 xyoos.
Hyaluronic acid invution (aqueous daim ntawv)
Cov kev daws teeb meem aeev ntawm ntau qhov kev cuam tshuam rau kev cuam tshuam ntau dua li cov hmoov pleev xim, feem ntau vim yog kev sib kis hydrolysis, thiab oxidation. Lawv feem ntau siv nyob rau hauv cov tshuaj pleev ib ce cov tshuaj pleev ib ce, ntshav qab zib, thiab cov khoom siv.
Cov Lus Qhia Cia Rau Ha Tshuaj:
• Ntsuas kub:
Qhov kev mob zoo tagnrho yog tub yees ntawm 2℃-8 degree. Khov yog poob siab, raws li cov dej khov siv cov dej khov tuaj yeem tsoo ha polymer chains thiab txo vepres.
• Kom tsis muaj menyuam:
Ha daws yuav tsum sterilized -}}} iradiation -}}}} Tshwj xeeb tshaj yog yog tias siv rau kev siv tshuaj lossis kho mob.
• Cov tshuaj txuag:
Hauv kev tshuaj pleev ib ce lossis tsis - Daim ntaub ntawv tsis muaj zog, cov tshuaj ntxuav tes, potassium sorbate, lossis phenoxyethanol yog siv los tiv thaiv kab mob kev loj hlob.
• Ntim:
HA kev daws teeb meem yuav tsum muab cia rau hauv cov av tsis huv, airtight ntim. Cov thawv ntim tsis pom zoo rau ntev - sij hawm cia vim yog ion txauv thiab ua haujlwm ES. Xaiv kev kho mob - qib plastics lossis coated iav.
• Txee lub neej:
Raws li kev cia khoom tsim nyog thiab nrog cov tshuaj tiv thaiv hauv cov tshuaj tiv thaiv hauv qhov chaw, cov tshuaj tseem ruaj khov rau 6-12 lub hlis. Txee lub neej yog qhov ua kom luv dua tsis muaj zog lossis tub yees.
Crosslinked lossis hloov hyaluronic acid
Crosslinked Ha feem ntau pom nyob rau hauv dermal fillers, kev cog lus, lossis cov gels kho mob. Los ntawm kev hloov kho tshuaj lom neeg (feem ntau nrog rau pawg hla (lwm cov saw hlau), ua rau lawv tiv taus cov kev ntxhov siab ib puag ncig thiab kev ntxhov siab ib puag ncig.
Cov lus qhia cia rau crosslinked ha:
• Ntsuas kub:
Chav nyob kub (15℃-25 Qib) feem ntau yog txaus rau crosslinked loj heev acid, tab sis cia hauv qhov kub- tswj ib puag ncig tseem tau qhia.
• Ntim:
Cov khoom no yuav tsum nyob hauv lawv cov khoom tsis muaj zog, kom tiv thaiv cov microbial paug. Zam kev qhib kom txog thaum siv.
• Txee lub neej:
Hla Ha cov khoom lag luam muaj lub neej txee ntawm 18-24 lub hlis, nyob ntawm cov txheej txheem tshwj xeeb thiab kev ntim kev ncaj ncees.
Yam cuam tshuam rau hyaluronic acid stability
Qhov kev ruaj ntseg ntawm hyaluronic acid (HA), txawm hais tias hauv cov hmoov lossis cov tshuaj tov, yog cuam tshuam los ntawm ntau ib puag ncig thiab tshuaj lom neeg. Cov xwm txheej no txiav txim siab ntev npaum li cas HA tswj nws cov molecular ncaj ncees, viscosity, thiab kev muaj nuj nqi. Hauv qab no yog cov lus qhia ntxaws ntxaws ntawm cov ntsiab lus tseem ceeb cuam tshuam rau Hyaluronic Acid muaj peev xwm.
Qho so
Qhov kub thiab txias ua lub luag haujlwm tseem ceeb hauv qhov tsis txaus ntseeg ntawm ntau cov tshuaj acidonic acid. Kev kub siab sim ua kom muaj kev sib tsoo ntawm HA cov saw hlau ntawm hydrolysis thiab thermal decomposition. Feem ntau:
Optimal storage temperature for HA is between 2℃and 10℃for long-term preservation.
Chav nyob kub (25 degree) tej zaum yuav tau txais rau luv- sij hawm cia tab sis tuaj yeem txo lub neej sib txawv dhau lub sijhawm.
High temperatures (>40 DEGREE) tuaj yeem ua rau kom nrawm nrawm thiab tsis pom lub homphosity ploj.
Kub -}}}}}}}}}}}}}}}}}}}} Txoj kev tsis txaus ntseeg tab sis kuj txo txoj kev ua haujlwm ntawm Ha nyob rau hauv cov kev cai ntawm hydration thiab elasticity.
PH-PHEEJ
Tej feem ntau cov tshuaj hyaluronic acid yog qhov ruaj khov nyob rau hauv me ntsis acidic rau nruab nrab p mob (kwv yees li PH.0-7.0). Kev sib txawv ntawm cov kab no tuaj yeem ua rau tsis muaj peev xwm:
• Acidic mob (pH <4.0) tej zaum yuav ua rau cov kua qaub - catalyzed hydrolysis, txo cov polymer saw ntev.
• Alkaline ib puag ncig (PH> 8.0) tuaj yeem ua kom -}} kev sib tua} kev sib tua, tawg hauv glycosidic khi hauv ha.
Yog li, tswj cov ntawv sib npaug hauv cov qauv yog qhov tseem ceeb heev rau lub sijhawm ntev Ha lub ruaj khov thiab ua haujlwm.
Yo noo thiab av noo
Yij yog ib tug tseem ceeb pab txhawb rau cov hydrolytic degradation ntawm ha, tshwj xeeb tshaj yog nyob rau hauv nws daim foos. Ntau ntau cov hyaluronic acid yog hygroscopic thiab tuaj yeem nqus tau cov dej noo huab cua, uas txhawb nqa:
• Kev nruab nrab ntawm cov tshuaj polysaccharide.
• Txo cov phaus molecular.
• clumping lossis noj ntawm cov hmoov, uas cuam tshuam rau kev ua thiab solubility.
To prevent this, HA powder should be stored in a dry environment with relative humidity below 30%, preferably in sealed, moisture-proof containers.
Lub teeb thiab UV kis
Hyaluronic acid yog rhiab rau ultraviolet (UV) hluav taws xob thiab ntev raug tshav ntuj, uas tuaj yeem:
• Txo cov saw polymer.
• Tsim cov roj oxygen cov pa (Ros) uas ua kom nrawm nrawm oxidative degradation.
• Ua kom muaj kev tsis sib haum xeeb thiab kev ua tau zoo hauv cov tshuaj pleev ib ce.
HA cov khoom yuav tsum tau ntim rau hauv opaque lossis UV {-} Cov thawv tiv thaiv thiab khaws cia ntawm lub teeb kom txo qhov kev pheej hmoo no.
Enzymatic degradation
Tej ntau hyaluronic acid tuaj yeem tawg los ntawm Hyaluronidase enzymes, uas yog ib txwm muaj nyob rau hauv lub cev thiab qee cov kab mob me me. Txawm hais tias tsawg kawg rau ha hmoov, enzymatic degradation yog qhov kev txhawj xeeb tseem ceeb hauv kev txhaj tshuaj tiv thaiv lossis cov ntawv thov cov ntawv thov. Cov neeg tswj hwm ruaj khov thiab cov tshuaj tiv thaiv feem ntau ntxiv rau cov qauv ua kom txo qis microbial kev loj hlob thiab kev ua si enzyme.
Daim ntawv ntawm ha
• Bulk Hyaluronic Acid Hmoov:
Ntau ruaj khov tshaj kev daws teeb meem vim tias tsis muaj dej, uas tiv thaiv hydrolysis thiab microbial kev loj hlob.
• Cov tshuaj aqueous:
Muaj kev cuam tshuam ntau rau hydrolysis, microbys me me, thiab oxidation, uas yuav tsum tau tswj cov khoom tshuaj thiab kev khaws cia.
Kev Tshawb Fawb Txog Hyaluronic Acid Cia
Kev tsis txaus siab hauv cov kev daws teeb meem aqueous ntawm ntau kub
A study examined bulk hyaluronic acid of varying molecular weights (14.3, 267.2, and 1,160.6 kDa) in pure water, stored at room temperature versus refrigeration for two months. Cov txiaj ntsig tau pom tias muaj kev poob phaus molecular phaus poob ntawm chav tsev kub (txog ~ 95%), thaum tub yees ua piv txwv tsis txhob ~ 5-8%. Qhov no qhia kom cov tub yees tub yees muaj qhov ua rau qeeb qeeb hauv Ha degradation hauv kev daws teeb meem. [1]
Ntev - lub sij hawm degradation ntawm ha hmoov
Kev tsom xam ntawm ntau cov hyaluronic acid khaws cia nyob rau hauv cov neeg sib txawv uas tau muab tso rau hauv qhov chaw nyob zoo dua (~ 5-10% ploj), tsis hais txog thaum pib molecular. [2]
Thermal Stability nyob rau hauv aqueous daim ntawv
Tej ntau cov tshuaj hyaluronic acid daws tau raug kuaj hla qhov kub ntawm 25℃thiab 100 degree. Txoj kev tshawb no siv viscosity hloov nyob rau hauv lub kaw ampoules los ntsuas kev ruaj khov. Siab dua qhov tsis muaj kev cuam tshuam HA lub zog rheological. [3]
Thermal degradation nyob rau hauv hmoov ha
Qhov kev tshawb fawb no tau soj ntsuam cov thermal depolymerization kinetics ntawm khoom sodium hyaluronate thoob rau PH theem. Depolymerization tau tswj hwm los ntawm kev ua kom random [4]
Kev ua rau ntawm PH siab thiab kub
Siab phiv nrog rau siab kub drastically Ib qho ntxiv, kev hloov pauv qauv yog kuaj tau ntawm FTIR tom qab 48 teev xwb. [5]
Oxidative Arection degradation
Rov qab cov pa oxygen - Xws li ozone, UV teeb, thiab ob peb txoj kev tshawb fawb pom tus nqi li cas degradation nyob ntawm molecular hnyav. [6]
Tsim kev ruaj nras nyob rau hauv Skincare
Kev kawm ruaj khov ntawm HA hauv cov duab kos duab ua yeeb yam tau nqa hata yauv dua li cov tshuaj pleev ib ce, vim yog hygroscopic cov khoom lag luam kom huv ruaj khov. [7]
Rheological stability ntawm ha -} cov qauv tsim
Dermal fillers hla -} Txuas nrog polyethylene protively profiles ntawm -20℃li 60 hnub, qhia tias muaj zog ntawm cov kev ntxhov siab. [8]
Hloov ha (thiol - ha "HA) kev ntsuas ruaj khov
Thiol {-}} hloov his (ha- Cysy (cua sov}}}}}}}}}}}}}}}}}}}}}}}}}}} Ob qho tib si qhia tau zoo heev nyob rau hauv tub yees; Lub HA CSYS Alla tseem ruaj khov thiab biocompible, tab sis cov kev hloov xim tau sau tseg hauv chav tsev kub. [9]
Tsim nyog cia ntawm ntau cov hyaluronic acid yog qhov tseem ceeb rau kev tswj cov khoom lag luam zoo thiab ua kom muaj txiaj ntsig cov khoom lag luam. Txawm hais tias nyob hauv cov hmoov, daws, lossis hyaluronic acid hmoov tsim kom cov kev tswj kom zoo los tiv thaiv nws qhov kub, av noo, thiab cov microbial hawv. Los ntawm kev ua raws li cov lus qhia cia kom pom zoo rau sab saud, cov neeg tsim khoom lag luam thiab cov neeg siv tuaj yeem ua kom muaj lub neej ntawm lub neej thiab kev ua tau zoo.
Guanjie Biotech, raws li tus kws tshaj lij kev kub siab acid xa khoom, kom txhua pob ntawm HIS ntsib cov qauv siab ntawm kev coj dawb huv thiab ruaj khov. Nrog cov chaw txawj ntse thiab cov kws tshaj lij, peb yog koj tus khub txhim khu kev qha hauv kev noj qab haus huv, kev zoo nkauj, thiab kws tshuaj. Txais tos rau nug nrog peb ntawm info@gybiotech.com.
Tuaj txog::
[1] Laurent, TC, & Frarer, JR (1992). Hyaluronic acid. Lub FaseB phau ntawv ceev xwm txheej, 6 (7), 2397-240-240.
[2] Weigel, Ph, Fuller, GM, & Lboeuf, Rd (1986). Tus qauv rau qhov kev ua tsis ncaj ntawm hyaluronic acid nyob rau hauv aqueous daws. Biopolymers, 25 (5), 953-968.
[3] Burdick, JA, & Prestwich, GD (2011). Hyaluronic acid hydrogels rau kev siv biomedical. Cov ntaub ntawv tshaj lij, 23 (12), H41-H56.
[4] Chaw Ua Si, S., li al. (2015). Kev ua rau ntawm hyaluronic acid nyob rau hauv ntau hom PH thiab cov xwm txheej kub. Polymers, 7 (7), 1497-1507.
[5] NECAS, J., li al. (2008). Hyaluronic acid (Hyaluronan): Kev tshuaj xyuas. Ericorinarni Medicina, 53 (8), 397-411.
[6] Q, X., li al. (2019). Kev tsis sib xws ntawm hyaluronic acid los ntawm alkaline thiab thermal kev nyuaj siab: UV - Vis thiab FTIR kev tsom xam thiab FTIR kev tsom xam thiab Ftir tsom xam. Phau ntawv Journal ntawm cov qauv molecular, 1194, 256-262.
[7] BACail, M., li al. (2022). Rheological thiab physicochemical tus cwj pwm tshiab tshiab ntawm cov tshiab peb 5}}} crosslinked hyaluronic acid {}}} rated dermal fillers. Gels, 8 (5), 264.
[8] Guarise, C., li al. (2020). Hyaluronic acid didadation los ntawm oxidative thiab thermal cov txheej txheem. Polymers, 12 (8), 1800.
[9] ARAúJO, VHS, li al. (2021). Tshiab cosmetic hydrogels raws li hyaluronic acid: physicochemical, rheological, thiab biocompatibility cim. Kws muag tshuaj, 14 (8), 767.
