Kuaj rauntshiab urolithin ib qho hmoovyog qhov tseem ceeb nyob rau hauv ntau thaj chaw, suav nrog kev kuaj mob hauv guanjie, thiab ua raws li cov khoom lag luam thoob ntiaj teb, encompassing qauv kev npaj, encompassing qauv kev npaj, encompining qauv kev npaj, encompining qauv kev npaj, encompassing qauv kev npaj, encompassing qauv kev npaj, encompassing qauv kev npaj, encompining qauv kev npaj, encompining qauv kev npaj, encompining qauv kev npaj, encompining qauv kev npaj, encompining qauv kev npaj, thiab cov txuj ci kev ntsuas, thiab kev ntsuas kev ntsuas, thiab kev ntsuas tshuaj kev paub tseeb kev cai .
Dab tsi yogUrolithin a?
Urolithin A yog cov metabolite tsim los ntawm kev hloov pauv ntawm ElligiS thiab Dibricial {{pyrones {} {pyrones rau nws cov txiaj ntsig kev noj qab haus huv, suav nrog anti-mob thiab cov khoom tiv thaiv kab mob antioxidant . Muab nws qhov tseem ceeb, txhim khu kev qha cov txheej txheem kev tshawb fawb thiab kev lag luam.
Qauv npaj rau urolithin kev sim
Ua ntej ib qho kev ntsuas kev tshawb xyuas, cov qauv kev ntsuas tsim nyog yog qhov tseem ceeb ua rau muaj cov hmoov sib cais xws li, cov hmoov roj ntsha, lossis cov nroj tsuag rho tawm.
EXTRACTION
Rau cov hmoov qauv, urolithin ib qho hmoov feem ntau tau muab tshem tawm lossis tshem tawm cov kuab tshuaj} feeb . Cov txheej txheem no pab ua kom muaj cov hmoov ua kom zoo nkauj
Kev piv txwv ntawm kev kuaj mob (cov zis, ntshav), muaj cov kua roj ua kua rau (lle) lossis lwm yam kev cuam tshuam)
Pom kev thiab centrifugation
After extraction, the sample is filtered using membrane filters (0.22 µm or 0.45 µm pore size) to remove particulates. Centrifugation ntawm 10, 000}}}} {}} rpm rau 10 feeb tej zaum yuav tau ua kom meej meej cov tshuaj.
Kev xav thiab rov ua dua
Yog tias tsim nyog, cov extract yog feeb kom txo qis siab siv cov ntsuas hluav taws xob qeeb nrog kev ntsuas me me, xws li mobile theem hauv HPLC.
Kev tshawb nrhiav cov tswv yim rau kev kuaj pom urolithin a
Kom ntseeg tau qhov tseeb thiab kom muaj tseeb ntawm urolithin yog qhov tseem ceeb rau kev tshawb fawb thiab kev ntsuas tshwj xeeb yog muaj rau Guanjie Biotech tus urolithin a Nrhiav tau .
Kev ua haujlwm siab ua kua chromatography (HPLC)
HPLC yog ib qho ntawm feem ntau ua haujlwm rau kev txheeb xyuas urolithin ib qho kev sib cuam tshuam nrog kev sib cais raws li kev sib cais thiab cov khoom siv mobile nyob rau hauv kev kub siab {}}
Kem: thim rov qab-theem C18 kem.
Mobile theem: feem ntau yog methanol-dej sib tov hauv 70:30 ntim piv rau {}}
Nrhiav kom tau: Ultraviolet (UV) Nrhiav tau ntawm 310.
Cov teeb tsa no tau pom dav dav rau nws lub peev xwm los muab kev sib cais zoo sib xws rau cov ntaub ntawv zoo sib xws rau cov ntaub ntawv zoo sib xws rau cov ntaub ntawv raw thiab ua tiav kev pab.
Ultra-kev ua tau zoo-kev ua kua chromatography (UHPLC)
USHPLC yog ib qho ntxiv siab dua ntawm HPLC, muab cov kev daws teeb meem sai sai uas ua rau muaj kev sib cais tshwj xeeb. nws tau siv cov hmoov kom zoo:
Cov theem mobile feem ntau suav nrog methanol-dej sib tov .
Tus txheej txheem ua tiav cov qauv zoo tshaj plaws thiab rhiab .
Cov txheej txheem tso cai rau qhov sib txawv ntawm urolithin a thiab nws lub peev xwm impurities .
Qhov no ua rau USPC zoo tagnrho rau high-toput expute ib puag ncig thiab niaj hnub ua kom muaj kev ntseeg zoo.
Kua kua chromatography-mass spectrometry (LC-MS)
LC-MS yog lub peev xwm hybrid muaj zog sib xyaw ua ke ntawm cov kua chromatography nrog lwm txoj kev yuav tsis rhiab txaus .
Kem: C18 thim rov qab-theem rov qab .
Mobile theem: Acetonitrile-dej sib tov nrog 0 .}} 1% formic acid.
Nrhiav kom tau txais: Mass Spectrometry siv los txheeb xyuas cov qauv molecular thiab concentration ntawm ntshiab urolithin hmoov .
Cov qauv no muaj cov kev xav tau zoo heev thiab muaj qee yam, ua nws tus qauv kub rau kev tshawb fawb pharmacokinate thiab bioavailability kev tshawb fawb {}}
Roj Chromatography-Pawg Spectrometry (GC-MS)
GC-MS feem ntau tau siv los kuaj cov urolithin ib qho tsis muaj suab paj nruag {1} Xav tau cov qauv sib xyaw ua ntej .
Tom qab kev hloov kho tshuaj lom neeg, ntshiab urolithin cov qauv hmoov yog txhaj rau GC-MS system .
Lub resulting loj pab txheeb xyuas thiab ntsuas kom meej impurities .
GC-MS yog cov txiaj ntsig zoo tshaj plaws hauv cov kev tswj kom zoo uas saib xyuas rau cov tshuaj paug thaum lub sijhawm tsim khoom thaum ntau lawm.
Ultraviolet-Pom (UV-Vis) Spectrophotometry
UV-Vis Spectrophhotometry yog txoj kev nrawm nrawm thiab kev nce qib rau kev ua ntej ntawm urolithin hmoov zoo.
Ua kom haum lub teeb nyob rau ntawm lub nthwv dej tshwj xeeb, feem ntau nyob ib ncig ntawm 310 nm .
Tus qauv kev nqus tau yog ntsuas thiab muab piv rau tus qauv nkhaus .
Txawm hais tias tsawg dua li Chromatographic cov tswv yim, UV-Vis yog pab tau rau kev kuaj nrawm lossis lees tias muaj cov qauv .
Nuclear Sib Nqus Revonance (NMR) spectroscopy
NMR yog cov cuab yeej tshawb xyuas qib siab siv los paub meej cov molecular tus kheej thiab cov qauv ntawm ua .
Nws qhia kom ntxaws spectra raws li hydrogen lossis carbon nuclei nyob rau hauv cov compound .
Nmr tuaj yeem txiav txim siab purity, txheej txheem kev ncaj ncees, thiab txawm pom cov hnub tso suab .
Vim nws muaj feem ntau, nmr feem ntau siv hauv kev tshawb fawb thiab kev lees paub kawg ntawm cov cim ntawm cov cim kev yog {}}
Kev tsim nyog ua kom zoo thiab tswj kev ua raws li kev ua raws
Ua kom muaj kev nyab xeeb thiab kev nyab xeeb ntawm urolithin cov khoom muaj hmoov los txheeb xyuas cov qauv kev coj ua zoo thiab kev nyab xeeb, thiab tag nrho.
Kev Kuaj Purity Purity
Kev kuaj mob huv si yog feem ntau siv cov khoom siv chratography (HPLC)
Kev Tshawb Xyuas Txhaum
Kev Txheeb Ze Puas yog lwm qhov tseem ceeb ntawm kev tswj kom tsis txhob muaj kev cuam tshuam (LC-MS) yog siv los xyuas cov tshuaj tsw qab (LC-MS) yog siv los kho cov tshuaj tsis huv
Cov Ntsiab Lus Kev Txiav Txim Siab
Cov ntsiab lus kev txiav txim siab cuam tshuam nrog kev siv UV-pom spectroscopy (necr) spectroscopy (N}} Cov txheej txheem sib xyaw ua ke, muab ib txheej ntxiv ntawm pov thawj.
Ua ke, cov qauv no ua kom txhua txoj kev ua kom zoo nkauj tsis suav nrog kev ua haujlwm zoo thiab kev nyab xeeb. {}
Cov txheej txheem kev ntseeg siab yog ib qho zoo, peb ua kom peb cov ntaub ntawv ua kom zoo nkaujinfo@gybiotech.com.
Kev tshuaj ntsuam rau urolithin ntshiab ib qho yuav cuam tshuam nrog cov qauv kev kuaj xyuas thiab cov qauv kev xav tau ua kom muaj kev nyab xeeb thiab ua kom muaj kev nyab xeeb ntawm kev nyab xeeb thiab ua tau zoo ntawm cov ntshiab Urolithin hmoov cov khoom hmoov, txhawb kev ntseeg siab ntawm cov neeg siv khoom thiab cov neeg muaj feem xyuam.
