Gynostemma Extract hmoov

Lus Taw Qhia

Gynostemma Extract hmoov Extract los ntawm gynostemma. Nws yog daj xim av zoo hmoov. Guanjie tsom rau tshuaj ntsuab extract rau 20 xyoo. Peb muaj kev paub dhau los. Peb siv txoj kev rho tawm: Grain cawv / dej. Peb siv HPLC txoj kev kuaj los kuaj peb cov khoom. Peb tuaj yeem muab cov ntawv tshaj tawm rau koj. Ntau tshaj 50 gibberellins raug cais tawm ntawm gibberellins. Thiab ginsenosides yog tib yam li ginsenosides. Nws belongs rau tib tetracyclic triterpenoid saponins. Nws yog cov uas gibberellins Ⅲ, Ⅳ, Ⅶ, Ⅻ yog tib yam li ginsenosides Rb1, Rb3, Rd, F2, gibberellin V-AH yog tib yam li ginsenoside Rg3. Gibberellin I yog tau los ntawm enzymatic decomposition ntawm ginsenoside K. Pharmacological thiab soj ntsuam decomposition ntawm ginsenoside K yog tau.

COA:

Flow Chart

Cov khoom xyaw:

Gypenosides (GPS), uas yog ib qho ntawm cov tshuaj nquag nquag ntawm gynostemma. Muaj ntau yam tshuaj muaj txiaj ntsig, uas tau ua rau gynostemma yog lub ntsiab lus tshawb fawb kub hauv xyoo tas los. Cov ntsiab lus ntawm saponins txawv ntawm gynostemma saponins, nrog gynostemma thiab gynostemma lanceolata muaj cov ntsiab lus saponin siab. Thiab gynostemma ntev-stem thiab gynostemma pentaphyllum muaj cov ntsiab lus saponin tsawg.

Basic Chemical Structure

Cov npuas tsim los ntawm kev co cov kua aqueous ntawm gynostemma extract hmoov tuaj yeem nyob tau ntev thiab tuaj yeem siv los txheeb xyuas saponins zoo. Saponins muaj polarity siab thiab yooj yim soluble nyob rau hauv dej kub, aqueous dilute ethanol, kub methanol thiab kub ethanol, thiab nyob rau hauv aqueous butanol thiab pentanol, thiab yog tsis tshua muaj soluble nyob rau hauv ether, benzene thiab lwm yam reagents ntawm tsawg polarity.

Cov khoom solubilization ntawm saponins tuaj yeem siv los cais cov saponins ntawm gibberellins thiab tau txais saponins crude. Lub melting point ntawm gibberellins yog siab, txog 139 degree mus rau 303 degree. Saponins tuaj yeem hydrolyzed los ntawm enzymes los tsim cov glycosides theem nrab, uas tuaj yeem ua rau cov saw hlau monosaccharide los yog hydrolyze cov saw bisaccharide rau cov saw hlau monosaccharide.

Acid hydrolysis hloov saponins mus rau theem nrab glycosides, uas yog ntxiv hydrolyzed los muab glycosides. Feem ntau ntawm cov tshuaj aqueous ntawm saponins tuaj yeem rhuav tshem cov qe ntshav liab thiab hemolytic.

Txoj kev rho tawm

Tam sim no, feem ntau siv gynostemma saponin extraction txoj kev yog ob qho tib si

1. Kev rho tawm dej cawv thiab dej rho tawm, ntxiv rau kev rho tawm lom neeg, txoj kev ultrasound, thiab lwm yam.

2. aAlcohol extraction method gibberellin tag nrho saponin tawm los yog siab dua, thaum cov dej extraction txoj kev yooj yim dua, ntau lwm yam tshuaj.

Gynostemma saponin extract decolorization thiab refining txoj kev

Gynostemma saponin extract yog xim dub thiab muaj ntau impurities, thiab nws tuaj yeem npaj ua zaub mov thiab dej haus uas muaj xim. Tab sis nws yog formulated nrog lub teeb-xim zaub mov thiab dej haus los yog rau cov hom phiaj tshuaj, nws yog tsim nyog los ntxiv decolorization thiab refinement ntawm gynostemma saponin extract kom tshem tawm pigments los yog impurities.

Tam sim no, muaj ntau txoj hauv kev rau decolorization thiab refinement ntawm gynostemma extracts, xws li hnyav extraction, resin method, resin-adsorbent method, dialysis method, thiab lwm yam. Tam sim no, ntau cov tswv yim ntau lawm yog txoj kev cob thiab sib tov.

Thaum kawm txog cov tshuaj pharmacological ntawm gibberellins, nws yog ib qho tsim nyog yuav tsum tau qhia meej txog cov ntsiab lus ntawm gibberellins thiab cais cov saponin ib leeg. Kev sib cais ntawm ib leeg-saponins feem ntau yog ua los ntawm kab chromatography.

Kev soj ntsuam zoo ntawm Total Saponins

1. Cov tshuaj tiv thaiv zoo Lippmann.

2. Cov tshuaj tiv thaiv zoo Sarkowski.

3.1 feem pua ​​aqueous tov yog nruab nrab, nrog nruam me npuas generated los ntawm co.

4. Acid hydrolysis ntawm insoluble feem yuav ntes ginseng diol.

5. UV nqus spectrum.

6. Infrared absorption spectrum.

7. Nyias txheej chromatography.

Kev ntsuas ntawm Total Saponins

Enzymes uas decompose gibberellins muaj nyob rau hauv tshiab gibberellins. Txhawm rau txiav txim siab cov ntsiab lus saponin ntawm cov qauv gynostemma tshiab, cov enzymes yuav tsum tau deactivated sai li sai tau los ntawm cua sov thiab ziab. Yog tias txoj kev ziab hauv qhov ntxoov ntxoo yuav ua rau ib feem ntawm saponin decomposition, qhov ntsuas tau qis. Feem ntau siv txoj kev txiav txim siab yog qhov hnyav thiab cov txheej txheem colorimetric, ntxiv rau nyias txheej chromatography colorimetric txoj kev, nyias txheej chromatography scanning txoj kev, thiab lwm yam.

Tag nrho cov ntsiab lus saponin ntawm gynostemma thiab nws cov yam ntxwv cuam tshuam

● Tag nrho cov ntsiab lus saponin ntawm gynostemma ntawm txawv keeb kwm

Feem ntau, cov ntsiab lus ntawm tag nrho cov saponins ntawm gynostemma, active ingredient rau cov hom phiaj tshuaj, yog siab. Txawm li cas los xij, qhov sib txawv ntawm tag nrho cov ntsiab lus saponin ntawm cov qauv ntawm cov cheeb tsam sib txawv yog qhov loj heev. Gynostemma spp. yog dav faib, thiab nws thaj chaw thiab ecological tej yam kev mob sib txawv. Yog li ntawd, tag nrho cov ntsiab lus saponin ntawm gynostemma txawv heev los ntawm cheeb tsam mus rau ib cheeb tsam. Txawm li cas los xij, nws tuaj yeem txiav txim siab tias tag nrho cov ntsiab lus saponin ntawm cov nroj tsuag gynostemma feem ntau yog li ntawm 5 feem pua ​​​​mus rau 10 feem pua ​​​​, thiab gynostemma nrog tag nrho cov ntsiab lus saponin ntau dua 10 feem pua ​​​​yuav siv tau los ua lub hom phiaj rau kev xaiv cov hom qoob loo siab.

● Qhov sib txawv ntawm cov ntsiab lus saponin ntawm ntau hom Gynostemma spp.

Qhov kev txiav txim siab ntawm tag nrho cov gibberellins ntawm ntau hom ntawm genus Gynostemma (feem ntau yog los ntawm txoj kev colorimetric, uas tseem sib piv) tau pom tias. Tag nrho cov ntsiab lus saponin ntawm Gynostemma rotundum thiab Gynostemma sparingly yog tsawg, ob qho tib si hauv qab 5 feem pua. Tam sim no, tus nqi tshuaj muaj tsawg. Gynostemma hirsutum, Burmese gynostemma, Guangxi gynostemma thiab ca-fruited gynostemma tag nrho muaj cov nqi siv ntawm cov gynostemma.

● Qhov sib txawv ntawm tag nrho cov ntsiab lus saponin ntawm cov kab mob sib txawv ntawm gynostemma

Hauv paus, qia, nplooj, paj thiab txiv hmab txiv ntoo hauv nruab nrog cev ntawm gynostemma muaj saponins. Txawm li cas los xij, cov ntsiab lus pom qhov sib txawv tseem ceeb, nrog cov ntsiab lus siab tshaj plaws nyob rau hauv nplooj, ua raws li cov txiv hmab txiv ntoo thiab qhov tsawg kawg nkaus hauv cov hauv paus hniav. Qhov kev faib ua feem ntawm saponins yuav txawv ntawm ntau theem fertility, tab sis tag nrho cov xwm txheej yog qhov ruaj khov.

● Tag nrho cov ntsiab lus saponin ntawm gynostemma txawv nyob rau lub sijhawm fertility sib txawv

Gynostemma tuaj yeem sau tau ob peb zaug hauv ib xyoos, tab sis tag nrho cov ntsiab lus saponin ntawm cov khoom tau sau nyob rau hauv ntau lub caij sib txawv, thiab lub sij hawm sau qoob loo zoo tshaj plaws tuaj yeem raug xaiv raws li cov kev hloov pauv hauv cov ntsiab lus saponin nrog lub sijhawm fertility. Cov txiaj ntsig ntawm gynostemma saponin cov ntsiab lus ntsuas nyob rau hauv lub sij hawm sib txawv ntawm fertility tsis zoo ib yam, tab sis qhov kev sib piv yog qhov zoo ib yam.

● Cov nyhuv ntawm lub teeb thiab kub thiab lwm yam ecological yam ntawm tag nrho cov ntsiab lus saponin ntawm gynostemma

Thaum lub teeb thiab kub tsis haum rau gynostemma fertility, nws yuav cuam tshuam rau tag nrho cov ntsiab lus saponin thiab tag nrho saponin sau (= tawm los (qhuav hnyav) × tag nrho cov ntsiab lus saponin). Tag nrho cov ntsiab lus saponin ntawm gynostemma tau nce nrog kev tshav ntuj nyob rau hauv qhov chaw kub (qhov nruab nrab txhua xyoo kub ntau dua lossis sib npaug li 15 degree).

● Cov nyhuv ntawm qhov siab ntawm tag nrho gynostemma extract hmoov cov ntsiab lus ntawm gibberellins

Qhov kev sib txawv ntsug ntawm cov huab cua hauv zos yog cuam tshuam los ntawm qhov siab sib txawv. Cov ntaub ntawv qhia tau hais tias tag nrho cov ntsiab lus saponin tau nce nrog qhov siab nce, tshwj xeeb tshaj yog qis dua 500 m, thiab tom qab nce mus rau qhov siab tshaj plaws, cov ntsiab lus tau nce mus rau tus nqi ruaj khov.

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